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Structural and functional neuroprotection in glaucoma: …

18/2/2010· Optic nerves were dissected, fixed in 2% osmium tetroxide, and eedded in epon resin. Semi-thin sections (0.7- μ m-thick) were cut on a microtome (Reichert, Vienna, Austria) and stained with 1%

Computational design of trimeric influenza-neutralizing

12/6/2017· The resin was washed with 50 mM Tris pH 8.0, 300 mM NaCl and 10 mM imidazole, and bound protein was eluted in the same buffer containing 250 mM imidazole. Proteins used in crystallographic or EM

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Reducing environmental risk by improving N management in

3/3/2009· After collection, the lysimeters were prepared for free drainage by replacing approximately 0.08 m of soil at the base of each column with a nylon mesh, a gravel layer, and a porous plastic sheet . The lysimeters were then placed in plots permanently installed belowground at the station. Each lysimeter was used to grow a waterlogged summer rice/upland winter …

The Ni-plated carbon fiber as a tracer for observation of …

Experimental measurements of fiber orientation are reported for two parts injection molded from nylon 6/6 reinforced with 43 wt% of glass fibers. The …

Proinflammatory doses of diesel exhaust in healthy …

All lavage samples were filtered through nylon (pore diameter 100 μm) and centrifuged at 400 g for 15 min. The supernatants were separated from the cell pellet and analysed for interleukin 6 (IL-6), IL-8, MPO and stem cell factor (SCF). IL-6, IL-8 and SCF were measured using commercially available ELISA kits (R&D Systems, Minneapolis Minnesota, USA). MPO was …

Airway antioxidant and inflammatory responses to diesel exhaust

Time-series studies demonstrate that short-term increases in aient particle concentrations are associated with hospital admissions 1 and deaths from respiratory and cardiovascular disorders 2, 3.In urban areas, motor vehicles can contribute to 25–35% of total airborne particulate matter (PM) with a diameter of <2.5 µm (PM 2.5) 4, 5, with concentrations near busy roads up to 30% …

US8789249B2 - Hidden-type convertible shoe - Google …

A shoe comprising a shoe upper including a male meer and a shoe sole including a receptor for locking with the male meer. The male meer locks with the receptor by moving the male meer with respect to the receptor in a first direction, which locks the shoe upper with the shoe sole. The male meer unlocks from the receptor by …

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(PDF) APPLIION OF CARBON FIBER REINFORCED …

The degree of resin impregnating into carbon fiber in CFRP is evaluated by analysing the range of the measured thermal diffusivity distribution. First, a reference material with known thermal

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Layered perovskite nanosheets bearing fluoroalkoxy

In recent years, layered perovskite-like oxides have attracted much attention because of their outstanding physical and chemical properties, including high-temperature superconductivity [1,2

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21/12/2021· Ms. Spurlin ASIX stock SEC Form 4 insiders trading. Sh has made over 6 trades of the AdvanSix Inc stock since 2016, according to the Form 4 filled with the SEC. Most recently she bought 11,765 units of ASIX stock worth $148,945 on 6 March 2020.. The largest trade she''s ever made was buying 11,765 units of AdvanSix Inc stock on 6 March 2020 worth …

Tooth Damage in Captive Orcas ( Orcinus orca ) | Request PDF

Request PDF | On Sep 1, 2017, John Jett and others published Tooth Damage in Captive Orcas ( Orcinus orca ) | Find, read and cite all the research you need on ResearchGate

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Teflon FEP. immersed in water, changes in relative humidity have little effect on the film. Figure 1: Tensile Stress vs. Elongation of Teflon™ FEP Film Figure 2: Tensile Properties of Teflon™ FEP Film vs. Temperature. 3 Teflon™ FEP Fluoropolymer Film Table 3.Typical Thermal Properties of Teflon™ FEP Film* Property ASTM Method SI Units English Units Melt Point D3418 (D TA) …

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Cloning and functional analysis of two gibberellin 3β - PNAS

3/7/2001· Studies have shown that GAs also regulate the development of reproductive organs. For example, a GA-deficient mutant in Arabidopsis, ga1–3, exhibits a male-sterile phenotype (), and in tomato stamenless-2 and gib-1 arrest anther development at an early stage (16, 17).In tall rice, it has been reported that GA 4 accumulates in anthers and that a cell-free extract from …

JCI - Obesity is associated with macrophage accumulation in …

15/12/2003· Obesity alters adipose tissue metabolic and endocrine function and leads to an increased release of fatty acids, hormones, and proinflammatory molecules that contribute to obesity associated compliions. To further characterize the changes that occur in adipose tissue with increasing adiposity, we profiled transcript expression in perigonadal

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Cloning and Structure-Function Analyses of Quinolone- and - JBC

Type III polyketide synthases (PKSs) synthesize various polyketide and alkaloid scaffolds.Results: QNS synthesizes quinolone as the single product, whereas ACS produces acridone as the major product.Conclusion: QNS and ACS are novel quinolone- and acridone-producing type III PKSs, respectively.Significance: Structure-function analyses of QNS and ACS provide insights into …

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Aliskiren ameliorates pressure overload-induced heart - Nature

7/7/2014· Aliskiren (ALK) is a renin inhibitor that has been used in the treatment of hypertension. The aim of this study was to determine whether ALK could ameliorate pressure overload-induced heart

A novel ligand for the NKG2D receptor activates NK cells and

27/1/2003· The Ig-fusion proteins were purified using a Ni 2+ resin (ProBond Resin; Invitrogen) following the manufacturer''s instructions. For flow cytometry analysis, cells were incubated for 30 min with the purified Ig-fusion proteins at a 1:50 dilution. After washing, the cells were incubated with a biotinylated mAb specific for the c-myc epitiope tag (9E10; Covance, Richmond, CA) …

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Functional Antagonism between CCAAT/Enhancer Binding Protein …

To remove steroid and thyroid hormones, fetal bovine serum was treated for 24 h at 4°C with 50 mg/ml activated charcoal and 30 mg/ml anion exchange resin (type AGX-8, analytical grade, Bio-Rad). After centrifugation, anion exchange resin was added again for 5 more hours. The resulting fetal bovine serum was spun again and filtered before use. 40-44 h after transfection the cells …

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6/2/2012· Nostalgic ice bag, easy to fill due to its big opening (capacity: 1,5 l), perfectly suitable for cooling sprains, pulled muscles, bruises etc. Comes in a …

Blood-Spinal Cord Barrier Alterations in Subacute and …

On the following day, the tissues were eedded in a fresh change of resin in tissue capsules. The blocks were polymerized at 70 °C in an oven overnight. The blocks were trimmed and then sectioned with a diamond knife on an LKB Huxley ultramicrotome. Thick sections cut at 0.35 µm were placed on glass slides and stained with 1% toluidine blue stain. Thin sections were cut at …